Mukharam, Khamdan (2025) Histomorphometric study of vasculoprotective effect of basil seed (ocimum basilicum) against long-term strenuous exercise in rattus norvegicus wistar strain / Khamdan Mukharam</p>. Diploma thesis, Universitas Negeri Malang.
Full text not available from this repository.Abstract
p Long-term strenuous physical exercise is a category of intense physical exercise performed regularly planned and aimed at improving fitness over an extended period. This training model has emerged in response to long-distance running competitions which require the body to adapt through modifications in intensity volume and frequency. Long-term strenuous physical exercise is an application of training that exceeds the recommended levels of physical activity and leads to increase in Reactive Oxygen Species (ROS) that disrupts the body s homeostatis. The effects include a decrease antioxidant defenses and increase in pro-inflammatory cytokines resulting in the risk of vascular damage as well cardiovascular disease this con dition presents an opportunity for the use of vasculoprotective agents through pharmacological approaches to protect the vasculature from inflammation. The objectives of this study include analyzing the effect of ethanol extract basil seed (Ocimum basilicum) on the histological features of the aorta of rats induced by long-term strenuous physical exercise and identification of effective doses on vascular damage indicator parameters. Identification of aortic damage due to long-term strenuous physical exercise includes the thickness of the tunica media the number of endothelial cells and the number of elastic lamellae and blood pressure as a supporting variable to the inflammatory mechanism. This research method uses a true experimental design with a complete randomized design and a posttest control only goup design research pattern using 25 white rats with inclusion criteria healthy rats weight 150 - 180 grams male sex and aged 2 - 3 months while exclusion criteria sick rats and rats that have lost weight more than 10% after adaptation. The treadmill exercise program or induction lasted for 8 weeks five days per week with intensity at 28 m/min for 60 minutes and incline of 10 . Ethanol extract Ocimum basilicum seeds was made by maceration method using 70% ethanol solvent and then evaporated to become a more concentrated extract. Rats were randomly divided into five sample groups with five heads in each group with KN (positive control) not induced exercise and given placebo KP (negative control) induced exercise and given placebo KA (Group A) induced exercise and given extract at a dose of 100 mg / kg bw KB (Group B) induced exercise and given extract at a dose of 200 mg / kg bw KC (Group C) induced exercise and given extract at a dose of 400 mg / kg bw. Data analysis using statistical tests of normality homogeneity and One Way Anova Test with Minitab 18 software. The rats were acclimatized for seven days and introduced to the treadmill. Following randomization each group underwent induction and treatment protocols for a duration of eight weeks. In the ninth week blood pressure was measured using the CODA non-invasive system for rats. After 24 hours the Morris Water Maze (MWM) test was conducted. The rats were then fasted for 8 hours anesthetized and subjected to surgical procedures for intracardiac blood collection and organ harvesting. The results of this study a significant difference in the thickness of the tunica media and the number of endothelial cells (especially the dose of 100 mg/kg BB) with a significance value (p lt 0.05) so it was concluded that the administration of the extract had an effect on histological changes in the aorta. Blood pressure profile data showed significant differences (especially the dose of 400 mg/kg BB) with significance (p lt 0.05) which means that the administration of the extract affects the blood pressure profile. However the number of elastic lamellae increased relatively with significance (P gt 0.05). These findings suggest that ethanol extract of Ocimum basilicum seeds has potential as a vasculoprotective agent against long-term strenuous physical exercise by improving histological structure and lowering blood pessure. /p
| Item Type: | Thesis (Diploma) |
|---|---|
| Divisions: | Fakultas Ilmu Keolahragaan (FIK) > Departemen Ilmu Keolahragaan (IK) > S1 Ilmu Keolahragaan |
| Depositing User: | library UM |
| Date Deposited: | 11 Jul 2025 04:29 |
| Last Modified: | 09 Sep 2025 03:00 |
| URI: | http://repository.um.ac.id/id/eprint/400604 |
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