Rahayu, Shinfa Auliya Ary (2025) Tumor protein-53 (tp53) expression in breast cancer and fibroadenoma mammae: a case study in RSAU dr. m. Salamun Bandung / Shinfa Auliya Ary Rahayu</p>. Diploma thesis, Universitas Negeri Malang.
Full text not available from this repository.Abstract
Breast cancer is the leading cause of cancer-related mortality among women worldwide including Indonesia which has recorded an increasing prevalence in the last five years. Breast cancer is a multifactorial disease caused by endocrinal and genetic factor. Different genes had been reported to be involved in tumorigenesis and/or further development. Had been widely known that Double Strand Break (DSB) causing breast cancer. TP53 is one among other genes responsible for DSB repair cell cycle regulation and apoptosis induction. This study aimed to analyse the possible involvement of TP53 gene in breast cancer and Fibroadenoma Mammae (FAM) tissues from patients at RSAU Dr. M. Salamun Bandung. A total of six breast tumour tissue including two samples of Invasive Carcinoma of No Special Type (grades II and III) and three FAM samples were analysed. TP53 gene expression was analysed using qPCR method. The expression level was calculated using 2- Ct relative quantification method and relative expression levels were compared among samples based on fold change values. The results showed that the highest expression was found in the grade II breast cancer sample followed by FAM while the lowest expression was observed in the grade III breast cancer sample. These findings suggest that the TP53 gene expression variation depends on tumour type and grade. Further studies with larger sample sizes specific mutation of TP53 and other tumour regulatory genes analyses as well as protein-level expression validation are needed to strengthen these findings for breast cancer cases in Indonesia. Keywords breast cancer fibroadenoma mammae gene expression molecular biomarker TP53.
| Item Type: | Thesis (Diploma) |
|---|---|
| Divisions: | Fakultas Matematika dan IPA (FMIPA) > Departemen Biologi (BIO) > S1 Bioteknologi |
| Depositing User: | library UM |
| Date Deposited: | 22 Aug 2025 04:29 |
| Last Modified: | 09 Sep 2025 03:00 |
| URI: | http://repository.um.ac.id/id/eprint/396037 |
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